Analytical Batch Verification

Every Peptide Spot batch is verified using high-performance liquid chromatography (HPLC) and electrospray ionization mass spectrometry (ESI-MS). Search our real-time Certificate of Analysis database below.

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≥99% HPLC Purity
Chromatograms on file
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ESI-MS Confirmed
Exact molecular mass
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ISO Standard QA
C18 RP-HPLC Protocol
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Batch Traceability
Lot-specific records
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Analytical Quality Process

How every research vial is synthesized, analyzed, and documented before release.

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Solid-Phase Synthesis

Synthesized via automated solid-phase peptide synthesis (SPPS) using high-grade Fmoc amino acid monomers.

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HPLC Purification

Preparative and analytical C18 RP-HPLC purification ensures all batches achieve >99% purity profile.

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Mass Spectrometry

ESI-MS / MALDI-TOF confirms exact molecular weight matching the calculated peptide mass within ±0.5 Da.

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COA Archival

Batch-specific analytical data is cataloged, verified by QA analysts, and attached to every shipped order.

Standard Specifications on Every Certificate

Custom Analytical Requests or Archival Inquiries?

Need historical batch data, high-resolution raw chromatogram CSV files, or institutional QA documentation? Our analytical team is available.

Contact Quality Assurance

Frequently Asked Questions

Are Certificates of Analysis included with every order?

Yes. Every shipment includes the batch-specific COA matching the lot number printed on your lyophilized vials.

What purity threshold does PeptideSpot enforce?

We maintain a strict minimum standard of ≥98% HPLC purity, with our average catalog batch testing between 99.7% and 99.9% purity.

How do I interpret the HPLC Chromatogram?

The HPLC chromatogram graphs optical absorbance (mAU) over time. A single sharp peak represents the purified peptide analyte, while the baseline integration demonstrates the absence of residual truncated peptide fragments.

How does Mass Spectrometry confirm peptide identity?

Electrospray Ionization Mass Spectrometry (ESI-MS) measures the mass-to-charge ratio (m/z) of the peptide. Comparing observed mass against calculated molecular weight confirms that the full-length amino acid sequence is synthesized correctly.

Research Use Only (RUO) All products and analytical data provided by Peptide Spot are intended strictly for laboratory in-vitro research and biochemical assays. Products are not drugs, foods, or medical devices and are not for human or veterinary use.